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  • Cat.No.
  • CSC-C3438
  • Description
  • Established from the adrenal tumor tissue resected after treatment from a 20-month-old boy of European origin with neuroblastoma (stage III) in 1991
  • Recommended Medium
  • 90% RPMI-1640 + 10% h.i. FBS + 2 mM L-glutamine
  • Organism
  • Human
  • Morphology
  • cell shape is bipolar, cells growing as monolayer or forming foci
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypotetraploid karyotype with 3.5% polypoidy - 88/89<4n>XXY/XXYY, -Y, -11, -17, -17, der(1qh++)t(1;17)(p34-35;q12)x2, t(6;9)(p21;q33)x2, ?inv(12)(q21q24)x2, der(22)t(17;22)(q21;p12)x2 - ca. 50-100 NMYC-containing dmin present in each cell - t(1;17)
  • Quality Control
  • Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament +, vimentin +
    Viruses: ELISA: reve
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • neuroblastoma

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com