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SH-SY5Y

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  • Cat.No.
  • CSC-C0329
  • Description
  • SH-SY5Y is one of three serially isolated neuroblast clones (SH-SY, SH-SY5, SH-SY5Y) of the human neuroblastoma cell line SK-N-SH which was established in 1970 from a metastatic bone tumor. The cells exhibit moderate levels of dopamine beta hydroxylase activity. They can convert glutamate to the neurotransmitter GABA. SH-SY5Y cells have a reported saturation density greater than 1 x 10^6 cells/cm2. The loss of neuronal characteristics has been described with increasing passage numbers (approx. passage 20). Neuronal markers or uptake of noradrenalin should be determined routinely. It is recommended not to use at passage numbers higher than 20 unless neuronal characteristics are controlled.
  • Recommended Medium
  • DMEM supplemented with 4.5g/L glucose, 2mM L-glutamine and 10% FBS.
  • Organism
  • Homo sapiens, human
  • Morphology
  • The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float; a confluent monolayer is not formed.
  • STR DNA Profile
  • Amelogenin: X
    CSF1PO: 11
    D13S317: 11
    D16S539: 8,13
    D5S818: 12
    D7S820: 7,10
    THO1: 7,10
    TPOX: 8,11
    vWA: 14,18
    D3S1358: 15,16
    D21S11: 31,31.2
    D18S51: 13,16
    Penta E: 7,11
    Penta D: 10,12
    D8S1179: 15
    FGA: 23.2,24
  • Culture Properties
  • Monolayer
  • Karyotype
  • modal number = 47; the cells possess a unique marker comprised of a chromosome 1 with a complex insertion of an additional copy of a 1q segment into the long arm, resulting in trisomy of 1q.
  • Source
  • Metastatic site: bone marrow
  • Quality Control
  • Tests for mycoplasma, bacteria and fungi were negative
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • Metastatic site: bone marrow
  • Application
  • The AGS cell line can be used for neurotransmitter studies , it can also be used as a suitable transfection host.

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com