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  • Cat.No.
  • CSC-C0455
  • Description
  • Established from a recurrent well-differentiated transitional papillary tumor of the urinary bladder (clinical stage T2, histological grade G1, treated with gold grains 2 years earlier and diathermy after recurrence 10 months later) of a 63-year-old man in 1968.
  • Recommended Medium
  • McCoys 5a medium supplemented with 2 mM L-glutamine and 10% FBS.
  • Organism
  • Homo sapiens, human
  • Morphology
  • epithelial
  • STR DNA Profile
  • Amelogenin: X,Y
    CSF1PO: 10,12
    D13S317: 8
    D16S539: 9
    D5S818: 11,12
    D7S820: 9,12
    THO1: 9,9.3
    TPOX: 8,11
    vWA: 14,17
  • Culture Properties
  • Monolayer
  • Karyotype
  • (P174) hyperdiploid and hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, translocations and minutes.
  • Source
  • transitional cell papilloma; bladder, urinary
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • transitional cell papilloma; bladder, urinary
  • Application
  • The RT-4 cell line can be used for tumourigenicity studies and can also be used as a suitable transfection host.

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Tel: 1-631-626-9181
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Email: info@creative-bioarray.com

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Tel: 44-207-048-3343
Email: info@creative-bioarray.com