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RT-112

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  • Cat.No.
  • CSC-C0268
  • Description
  • Established from the transitional cell carcinoma (histological grade G2) excised from a woman (age unknown) with untreated primary urinary bladder carcinoma in 1973; described to form tumors in nude mice. Cytokeratine (4),5,(6), 7, 8, 13, 17, 18, 19, Desmoplakin; DNA-index=2,1.
  • Recommended Medium
  • RPMI-1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum.
  • Organism
  • Homo sapiens (human)
  • Morphology
  • Epithelial
  • STR DNA Profile
  • Amelogenin: X,X
    CSF1PO: 10,11
    D13S317: 13,14
    D16S539: 11,13
    D5S818: 10,13
    D7S820: 12,11
    THO1: 7
    TPOX: 8,11
    vWA: 14,17
    D3S1358: 15
    D21S11: 27,30
    D18S51: 15
    Penta E: 12,16
    Penta D: 10,11
    D8S1179: 13,15
    FGA: 23
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hyperdiploid karyotype with 4% polyploidy-47(44-47)<2n>XX/XXX, +X, +10, +11, +14, -17, +mar, add(2)(q3?2), del(3)(p14p24.1), i(4p), add(6)(q2?), i(8q), del(9)(p21p22), add(10)(p12), der(11)t(3;11)(q11-13;p15), add(17)(p1?1), add(18)(q11-22)-side.
  • Source
  • Urinary bladder
  • Quality Control
  • Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
    specific PCR: negative
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • Urinary bladder

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com