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Porcine Small Intestinal Epithelial Cells

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Cat.No.
CSC-C9253J
Description
Porcine Small Intestinal Epithelial Cells from Creative Bioarray are isolated from small intestinal tissue of porcine. Porcine Small Intestinal Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Species
Porcine
Source
Intestine
Recommended Medium
Complete Epithelial Cell Medium
Cell Type
Epithelial
Disease
Normal
Storage and Shipping
We ship frozen cells on dry ice. Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
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Q:
How to eliminate contamination of tissue culture?
A:

When significant culture contamination occurs, the investigator may attempt to eliminate or control the contamination. First, determine whether the contaminant is bacterial, fungal, mycoplasma or yeast, isolate the contaminated cells from other cell lines, disinfect culture vessels and ultra-clean tables with laboratory disinfectant, and check HEPA filters. High concentrations of antibiotics and antimycotics may be toxic to some cell lines, and thus, dose-response experiments are done to determine the dose levels at which antibiotics and antimycotics produce toxicity. This is especially important when using antibiotics such as amphotericin B and antimycotics such as tylosin.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Subtlety

The goods we receive correspond to the list, and they have not made any mistakes in this regard.

12 Apr 2023


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