Porcine Pancreatic Microvascular Endothelial Cells

Specification
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Cat.No.

CSC-C8654W

Description

Porcine Pancreatic Microvascular Endothelial Cells from Creative Bioarray are isolated from pancreatic tissue of porcine. Porcine Pancreatic Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.

Species

Porcine

Source

Pancreas

Cell Type

Endothelial

Disease

Normal

Quality Control

Porcine Pancreatic Microvascular Endothelial Cells are tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Porcine Pancreatic Microvascular Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray.Repeated freezing and thawing of cells is not recommended.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

Why does the culture medium become dark red in color and increasingly alkaline in pH when stored in a 4 C refrigerator?

When the medium is stored in a 4°C refrigerator, the CO2 in the medium will gradually escape, causing the medium to become more alkaline, and the color of the acid-base indicator (usually phenol red) in the medium will become more dark red as the alkalinity increases. The result of alkaline medium will cause cell growth stagnation or death. If the medium is alkaline, the pH can be adjusted by introducing sterile filtered CO2.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Long performance life

The cell product has long performance life.

10 Feb 2023

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