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Porcine Carotid Artery Endothelial Cells
- Specification
- Recommended Products
Cat.No.
CSC-C8633W
Description
Porcine Carotid Artery Endothelial Cells from Creative Bioarray are isolated from carotid artery of porcine. Porcine Carotid Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Species
Porcine
Source
Artery
Cell Type
Endothelial
Disease
Normal
Quality Control
Porcine Carotid Artery Endothelial Cells are tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Porcine Carotid Artery Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What are the advantages of sorting with magnetic beads?
A:
Screening of endothelial cell-specific antigen PECAM with antibodies coupled to paramagnetic magnetic beads separates pure endothelial cells from samples contaminated with non-endothelial cells.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Well done
This sorting method has helped us save a lot of cells.
02 Feb 2023
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