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NCI-H548

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C9537L
  • Description
  • Species: human - male, 52 years old, Caucasian
    Tumorigenecity: yes, in nude mice
    Isoenzyme: G6PD, B; PGM1, 1 ;PGM3, 1;ES-D, 1;AK-1, 1-2;GLO-1, 2
    Production: carcinoembryonic antigen, 18ng/ml per 10 exp6 cells per 10 days
    Histopathology: adenocarcinoma
    Note: This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985. The line was obtained from a well differentiated sigmoid tumor from a patient prior to therapy. The line was initially grown in medium with fetal bovine serum, but was later adapted to growth in the chemically defined medium ACL-4. Floating aggregates produce tubuloglandular structures lined by columnar epithelia. When grown in medium with serum, the cell attach partially to the substrate and form domes. The cells and tumors formed from the cells are positive for Dopa decarboxylase and are negative for the TAG-72 antigen
  • Recommended Medium
  • ACL-4 medium with modifications
  • Organism
  • Human
  • Morphology
  • epithelial
  • STR DNA Profile
  • D3S1358: 16
    vWA: 15,18
    FGA: 21,22
    Amelogenin: X
    TH01: 7
    TPOX: 10,11
    CSF1P0: 11
    D5S818: 12,13
    D13S317: 9,11
    D7S820: 9,11
  • Culture Properties
  • floating aggregates of round cells with some attached cells
  • Karyotype
  • the Y chromosome is not present in most of the cells
  • Source
  • colon
  • Quality Control
  • Tests for mycoplasma, bacteria and fungi were negative
  • Storage and Shipping
  • Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com