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NCI-H295R

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C8887H
  • Description
  • NCI-H295R was adapted from the NCI-H295 pluripotent adrenocortical carcinoma cell line established by A.F. Gazdar and associates (1990) from a carcinoma of the adrenal cortex. The original cells were adapted to a culture medium which decreased the population doubling time from 5 days to 2 days. While the original cells grew in suspension, the adapted cells were selected to grow in a monolayer. This cell line retains the ability to produce adrenal androgens. It is responsive to angiotensin II and potassium ions.
  • Recommended Medium
  • DMEM/Ham's F12 (1:1 mixture) containing 15 mM HEPES, 0.00625 mg/ml insulin, 0.00625 mg/ml transferrin, 6.25 ng/ml selenium, 1.25 mg/ml bovine serum albumin, and 0.00535 mg/ml linoleic acid and 2.5% Nu-Serum I.
  • Organism
  • Homo sapiens ,human
  • Morphology
  • epithelial
  • STR DNA Profile
  • Amelogenin: X,X
    CSF1PO:10,12
    D13S317: 13
    D16S539: 11
    D5S818: 12
    D7S820: 9,12
    TH01: 9.3
    TPOX: 8
    vWA: 17,18
    D3S1358: 15,16
    D21S11: 32.2
    D18S51: 17
    Penta E: 5,12
    Penta D: 8
    D8S1179: 13
    FGA: 19.2, 24
  • Source
  • adrenal gland
  • Quality Control
  • Tests for mycoplasma, bacteria and fungi were negative
  • Storage and Shipping
  • liquid nitrogen vapor phase

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
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      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com