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Mouse Embryonic Stem Cell-derived Endothelial Cells

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C4207X
  • Description
  • Mouse Embryonic Stem Cells (MSCs) are isolated from day 3.5 (E3.5) blastocyts of pathogen-free laboratory mice. The established ES cell lines are propagated by co-culture with mouse embryonic fibroblasts (MEF) as described in standard mouse ESC culture protocol. Mouse Embryonic Stem Cell-Derived Endothelial Cells (ES-ECs) from Creative Bioarray are isolated from MESCs. ES-EPCs are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Creative Bioarray's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.
    ES-ECs can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
  • Species
  • Mouse
  • Recommended Medium
  • Complete Mouse Endothelial Cell Culture Medium
  • Organism
  • Mouse
  • Source
  • Embryonic
  • Storage and Shipping
  • Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.

    Never can primary cells be kept at -20 °C.
  • Cell Type
  • Endothelial Cells

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com