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  • Cat.No.
  • CSC-C0448
  • Description
  • Originated from surgical material of a human breast carcinoma; the tumor was passaged in nude mice prior to adaptation to continuous growth in culture vessels
  • Recommended Medium
  • 90% RPMI-1640 + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate
  • Organism
  • Human
  • Morphology
  • epithelial cells, after splitting cells start to grow in colonies (not necessarily as monolayer)
  • Karyotype
  • Human hyperdiploid karyotype with 8% polyploidy - 47(45-48)<2n>X, -X, +7, +22, i(13q), del(22)(q11) - trisomy 7 recurrent in breast cancer - resembles published karyotype
  • Quality Control
  • Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokerati
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 3.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • colon carcinoma

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com