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MDA-MB-231

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  • Cat.No.
  • CSC-C8210L
  • Tissue of Origin
  • Breast
  • Description
  • This cell line was established from a pleural effusion of a 51 year old woman with metastatic breast cancer.
    Tumorigenecity:Yes, in nude mice also in ALS treated BALB/c mice; forms poorly differentiated adenocarcinoma(grade III)
    Isoenzyme:Me-2,1-2;PGM3,1;PGM1,1-2;ES-D,1;AK-1,1;GLO-1,2;G6PD B
    Histopathology:adenocarcinoma; pleural effusion
    Subculture:Remove medium, rinse with fresh 0.25 % trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37ÂșC).
  • Recommended Medium
  • DMEM:Ham's F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine and 5% fetal bovine serum.
  • Organism
  • Human
  • Morphology
  • epithelial
  • STR DNA Profile
  • Amelogenin: X,X
    CSF1PO:12,13
    D13S317:13
    D16S539:12
    D5S818:12
    D7S820:8,9
    TH01:7,9.3
    TPOX:8,9
    vWA:15,19
    D3S1358: 16
    D21S11: 30,33.2
    D18S51: 11,16
    Penta E: 11
    Penta D: 11,14
    D8S1179: 13
    FGA: 22,23
  • Culture Properties
  • Adherent
  • Karyotype
  • mean chromosome number = 68; (P17), hypotriploid with abnormalities including breaks, secondary constrictions and fragmentations.
  • Source
  • Breast; mammary gland; (pleural effusion)
  • Quality Control
  • Tests for mycoplasma, bacteria and fungi were negative
  • Storage and Shipping
  • liquid nitrogen vapor phase
  • Tissue
  • Breast; mammary gland; (pleural effusion)

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Tel: 1-631-626-9181
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Email: info@creative-bioarray.com

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Tel: 44-207-048-3343
Email: info@creative-bioarray.com