The MCF-7 cell line was established from the pleural effusion of a patient suffering from a breast adenocarcinoma. It retains several characteristics of differentiated mammary epithelium including the ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. Growth of MCF-7 cells is inhibited by tumor necrosis factor alpha (TNF alpha). Secretion of IGFBP's can be modulated by treatment with anti-estrogens.
EMEM supplemented with 2mM L-glutamine, sodium pyruvate, NEAA and 10 % FBS.
The stemline chromosome numbers ranged from hypertriploidy to hypotetraploidy, with the 2S component occurring at 1%. There were 29 to 34 marker chromosomes per S metaphase; 24 to 28 markers occurred in at least 30% of cells, and generally one large submetacentric (M1) and 3 large subtelocentric (M2, M3, and M4) markers were recognizable in over 80% of metaphases. No DM were detected. Chromosome 20 was nullisomic and X was disomic.
Tests for mycoplasma, bacteria and fungi were negative
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liquid nitrogen vapor phase
The MCF-7 OS cell line can be used as a suitable transfection host.
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