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LN-405

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  • Cat.No.
  • CSC-C0317
  • Description
  • Glioblastoma cells established from an astrocytoma tumor (grade IV) of a 62-year-old woman in 1986; cells were described as expressing mRNA for both TGF-β1 and G-TSF/TGF-β2 (but only the latter polypeptide was secreted) and expressing GFAP protein
  • Recommended Medium
  • 90% DMEM + 10% h.i. FBS
  • Organism
  • Human
  • Morphology
  • fibroblastic, grows as monolayers in culture
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypertetraploid karyotype with 37% polyploidy and with 1-3 supernumerary dm present in most cells - 102-108<4n>XXXX, +1, +2, +3, +4, +5, +7, -10, +11, +13, -16, -16, -16, +19, +20, +20, +21, +22, +8mar, del(1)(q41)x2, der(1)t(1;3)(p32;p11), add(4)(p
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • astrocytoma

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

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Tel: 44-207-048-3343
Email: info@creative-bioarray.com