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KYSE-270
- Specification
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Cat.No.
CSC-C0428
Description
Derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 79-year-old Japanese man prior to treatment (depth of invasion was not beyond the muscularis propria); described to carry p53 mutation
Species
Human
Source
esophageal squamous cell carcinoma
Recommended Medium
Culture Properties
monolayer
Morphology
epitheloid cells growing in monolayers
Karyotype
Human hypotriploid karyotype with 16% polyploidy - 66(64-67)<3n>XX/XXX, +1, -2, +3, -5, -6, +7, +8, -9, -10, -15, -15, -17, -18, -19, -20, -22, +5mar (1bisat), ?del(X)(q25), add(1)(p1?), add(1)(p2?), i(1p), der(1)t(1;?)(?;2)(q13;?)(?;q11), add(2)(q37), de
Quality Control
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 - , cytokeratin-8 +, cytokeratin-17 (+), cytokeratin-18 +,
Immunology: cytokeratin +, cytokeratin-7 - , cytokeratin-8 +, cytokeratin-17 (+), cytokeratin-18 +,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What are the consequences of using too much serum?
A:
Too much serum tends to change the cells in culture, especially some diploid infinite cell lines, which are prone to malignant transformation after rapid growth.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Professional and accommodating
The staff in the Creative Bioarray were incredibly professional and accommodating. They went above and beyond to make sure I was satisfied with the services provided.
18 July 2023
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