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KU-19-19

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  • Cat.No.
  • CSC-C0442
  • Description
  • Established from the invasive transitional cell carcinoma of the bladder (grade 3, pT3b) of a 76-year-old man in 1993; described as secreting several hematopoietic cytokines and chorionic gonadotropin
  • Recommended Medium
  • 90% RPMI-1640 + 10% h.i. FBS
  • Organism
  • Human
  • Morphology
  • epithelial-like cells growing adherently in monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypertriploid karyotype - 74(70-75)<3n>XXX, +1, +5, +6, -7, +9, -11, +12, +15, +16, -17, -18, +19, +21, -22, +2mar - add(1)(p32-34)/del(1)(p34), add(4)(q35)x1-2, del(5)(q14.2), add(6)(q11), del(11)(p14), add(12)(q24)/der(12)t(12;17)(q24;q12-22), add
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vim
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • urinary bladder transitional cell carcinoma

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com