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KPL-1

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  • Cat.No.
  • CSC-C0389
  • Description
  • Established from the pleural fluid of a 50-year-old Japanese woman with ductal carcinoma of the breast with a predominant intraductal component in 1992; cells were described to express estrogen, but not progesteron receptors and to secrete tissue polypeptide antigen; however, DNA fingerprinting analysis at the Creative Bioarray showed cross-contamination with cell line MCF-7; MCF-7 was established from the pleural effusion of a 69-year-old Caucasian woman with metastatic mammary carcinoma
  • Recommended Medium
  • 90% DMEM + 10% h.i. FBS
  • Organism
  • Human
  • Morphology
  • adherent growing in monolayers (very dense colonies)
  • Culture Properties
  • monolayer
  • Karyotype
  • Human highly rearranged hypertriploid karyotype with 4% polyploidy - 77(70-82)<3n>XX, -X, -1, +2, -11, -13, +14, +15, -17, -18, -19, -20, -20, -21, -22, +15mar - add(X)(q28), add(2)(q35), add(3)(p25)x2, add(5)(p15), add(7)(p14), add(7)(p14), add(8)(p12),
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • breast carcinoma

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com