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KB-3-1

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C6211X
  • Description
  • The parental continuous cell line HELA was established from the primary cervix carinoma of a 31-year-old black woman 1951; KB-3-1 is a derivative of HELA and is used in multiple drug resistance studies giving raise to drug-resistant mutants (e.g. KB-V1 resistant to vinblastine; KB-CHR-8-5 resistant to colchicine); cells express MYC mRNA; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license at the NIH/NCI Technology Transfer Cernter, Rockville, MD, USA, phone (301)435-3111
  • Recommended Medium
  • 90% DMEM + 10% h.i.FBS + 1 mM sodium pyruvate
  • Organism
  • Human
  • Morphology
  • epithelial-like, adherent cell line growing in monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypertriploid karyotype with 1.8% polyploidy - 76(73-78)<3n>XX, -X, -1, -3, -4, +6, +7, +8, -9, -18, -20, +11mar, der(1)t(1;?)((p11;?), der(3)t(3;?)(p11;?), der(3) t(3;?)(p14;?), der(3p;5q), del(6)(q21), del(7)(q31), der(8)t(8;?11) (q24;q11), der(9)
  • Source
  • cervix carcinoma (derivative of HELA)
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
    Viruses: ELISA
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com