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JMSU-1

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  • Cat.No.
  • CSC-C0515
  • Description
  • Derived from malignant ascitic fluid of a 75-year-old man with urinary bladder carcinoma in 1988; described as being of epithelial origin with morphologically distinct cells (polygonal to spindle-shaped) and as expressing cytokeratin-19 fragment
  • Recommended Medium
  • 90% RPMI-1640 + 10% h.i. FBS + 2 mM L-glutamine
  • Organism
  • Human
  • Morphology
  • adherent epitheloid cells growing in monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Human flat-moded hypertriploid karyotype with 12% polyploidy - 69-74<3n>X/XX, -Y, -Y, +1, +1, +2, -5, +9, -12, +13, -15, -15, -16, -17, +19, -22, +9mar, add(1)(q32), add(2)(q3?), del(4)(q31q33), del(6)(q25), add(7)(p11), del(8)(p22), del(9)(p22), del(10)(
  • Quality Control
  • Mycoplasma: negative in microbiological culture, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
    Viruses: PCR: EBV -, HBV -, HCV -, HIV
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • urinary bladder carcinoma

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com