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JIMT-1

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  • Cat.No.
  • CSC-C0592
  • Description
  • Established from the pleural effusion of a 62-year-old woman with ductal breast cancer (grade 3 invasive, T2N1M0) after postoperative radiation in 2003; cell line was described to carry an amplified HER-2 oncogene and to be insensitive to HER-2-inhibiting drugs, e.g. trastuzumab (Herceptin)
  • Recommended Medium
  • 90% DMEM + 10% h.i. FBS
  • Organism
  • Human
  • Morphology
  • epithelial-like cells growing in monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Human flat-moded highly rearranged hyperdiploid karyotype with 6% polyploidy - 46-56<2n>XX, +1, +2, +2, +3, +5, +6, +7, +12, -13, -14, -15, -15, -16, -18, +17, +5mar, add(X)(p22.1), add(1)(q11), del(1)(q32.2), der(1)add(1)(p21)add(1)(q22), der(1)t(1;6)(p1
  • Quality Control
  • Mycoplasma: negative in microbiological culture, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
    Viruses: PCR
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • breast carcinoma

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com