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Human Preadipocytes-subcutaneous

  • Specification
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Cat.No.
CSC-7736W
Description
Adipocytes play an important role in energy storage and metabolism. Adipocyte differentiation is a developmental process that is critical for metabolic homeostasis and nutrient signaling. It is controlled by complex actions involving gene expression and signal transduction. Preadipocytes are present throughout adult life in adipose tissues and can proliferate and differentiate into mature adipocytes according to the energy balance. The proliferation and differentiation of these preadipocytes contribute to increases in adipose tissue mass. In vitro study indicates that different tissue-derived preadipocytes exhibit differently in lipid accumulation, adipogenic transcription factor expression, and TNF?-induced apoptosis. It has also been demonstrated that there is a close relationship between adipocyte differentiation and many physiological and pathological processes including fat metabolism, energy balance, obesity, diabetes, hyperlipidemia and breast cancer. HPA-s from Bioarray Research Laboratories are isolated from human subcutaneous fat tissue. HPA-s are cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HPA-s are characterized by immunofluorescent method with antibodies to CD44, CD90 and lipid staining after differentiation. HPA-s are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPA-s are guaranteed to further culture at the conditions provided by Bioarray Research Laboratories.
Species
Human
Source
Adipose
Cell Type
Preadipocyte
Disease
Normal
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
  • Customer Review
Q:
Where are the cells from?
A:

The preadipocytes are isolated from human subcutaneous adipose tissue. The adipocytes are differentiated in vitro using these isolated preadipocytes.

Q:
Can I pass the cells?
A:

Preadipocytes can be trypsinized and replated several times. Preadipocytes grow slower with each passage and differentiate poorly after passage 4. Cells are shipped at Passage 2-4.

Q:
How to differentiate human preadipocytes?
A:

You can order preadipocytes and pre-made culture media kit (cat# CM-1145X) for adipocyte differentiation. The protocol for differentiating the cells can be found in our manual.

Q:
Can I request cells from specific depots?
A:

Yes, we often have cells from various depots available. Please inquire as to price and availability.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Easy recovering

During our experiments, the cell product was easily recovered.

19 May 2022


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