Human Mesenchymal Stem Cells provide an ideal culture model for the study of multipotent stem cell biology. HMSC-Ad, HMSC-WJ and HMSC-Pre-Adipocyte can each be expanded in an undifferentiated state for future differentiation to multiple lineages.
HMSC may be differentiated down the typical mesenchymal lineages, such as adipogenic, chondrogenic, and osteogenic lineages. HMSC are cryopreserved as secondary cells to ensure optimal phenotype and the highest viability and plating efficiency. Our HMSC are quality tested via flow cytometry to ensure proper expression of multiple markers of mesenchymal stem cells. There is a consensus in the published literature that mesenchymal stem cells do not express hematopoietic surface proteins such as CD45, CD34, and CD14, and that they do express STRO-1, SH-2 (CD105, endoglin), and SH3/SH4 (CD73). However, quantification of positive vs. negative expression is not universally standardized. CB® has set stringent parameters for quantification of marker expression. HMSC are uniformly positive for integrin CD29 matrix receptors CD44 and CD105 and stromal cell-associated markers CD73, CD90, and CD166. HMSC are uniformly negative for hematopoietic lineage markers CD14, CD31, CD34, and CD45.
Homo sapiens, human
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liquid nitrogen vapor phase
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