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Human Mammary Fibroblasts

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  • Specification
  • Review & FAQ
  • Recommended Products
  • Cat.No.
  • CSC-C1500
  • Description
  • Fibroblasts are the most common types of mammary stromal cells. Mammary stromal cells differentiate into fibroblasts, adipocytes or vascular structures in a hormone- and substatum-dependent manner, and may explain the dramatic changes in stromal composition during both normal mammary gland development and tumorigenesis. The interaction between stromal cells and tumor cells is known to play a major role in cancer growth and progression. The Mammary stromal fibroblasts express and produce cytokines such as SF witch plays roles in mammary cancer.
    HMF are isolated from human mammary tissue. HMF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HMF are characterized by immunofluorescent method with antibodies to fibronectin, CD90 and vimentin. HMF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMF are guaranteed to further expand for 15 population doublings at the conditions provided by Creative Bioarray.
  • Species
  • Human
  • Recommended Medium
  • It is recommended to use Fibroblast Medium for the culturing of HMF in vitro.
  • Organism
  • Human
  • Morphology
  • Fibroblasts
  • Quality Control
  • Human Primary Mammary Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Human Primary Mammary Fibroblasts are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray.Repeated freezing and thawing of cells is not recommended.
  • Storage and Shipping
  • We ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
    Never can primary cells be kept at -20 °C.
  • Tissue
  • Breast
  • Application
  • Human Primary Mammary Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
  • Cell Type
  • Fibroblasts

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Contact Us
    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com