Human Calvarial Osteoblasts (HCO)

Specification
Recommended Products

Cat.No.

CSC-7820W

Description

Human Calvarial Osteoblasts (HCO) from Creative Bioarray are isolated from human calvariae. HCO are cryopreserved at primary cultures and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCO are characterized by the cytochemically detection of AP and mineral deposition. HCO are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCO are guaranteed for 15 population doublings at the conditions provided by Creative Bioarray.

Species

Human

Source

Bone

Cell Type

Osteoblast

Disease

Normal

Storage and Shipping

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CSC-7744W

Human Osteoblasts-femural (HO-f)

INQUIRY

CSC-7860W

Human Osteoblast Cells

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review

How would you describe the morphology of Human Osteoblast?

Creative Bioarray’s osteoblasts generally appear fibroblastic. They are cuboidal in lower densities and become spindle shaped as they become confluent.

What is the purity of our osteoblasts?

It is not easy to give an exact number for the purity of osteoblasts as the tests used are all functional assays: alkaline phosphatase production and bone mineralization, which unless you have a synchronized culture, not all the cells are doing the same thing at the same time. Based on the cell morphology and other parameters, we consider the purity of the osteoblast culture to be approximately 95%.

What media should I use?

It is recommended to use SuperCult® Osteoblast Growth Medium Kit (cat# CM-1149X) for the culturing of primary human osteoblasts.

Can anyone share a good method for quantifying alizarin red staining in osteoblasts?

You can either use chemical elution or digital image quantification. Based on Alizarin Red's fluorescence properties, fluorescence spectroscopy might work, as well. No matter which tool you are going to use, make sure that your pH is well calibrated. Otherwise your dye will change colour between yellow, red and purple.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Perfect

These cells are of high quality and allow reproducible results up to numerous passages.

09 July 2022

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