Human Bone Marrow-Derived Endothelial Cells

Specification
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Cat.No.

CSC-C8593W

Description

Human Bone Marrow-Derived Endothelial Cells from Creative Bioarray are isolated from human bone marrow tissue. Human Bone Marrow-Derived Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

Species

Human

Source

Bone Marrow

Cell Type

Endothelial

Disease

Normal

Quality Control

Human Bone Marrow-Derived Endothelial Cells from Creative Bioarray display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody CD31 (Catalog No. 550389, BD; CD31/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R&D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-5 passages under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

What method is used for the isolation of primary endothelial cells?

The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are isolated with anti-PECAM-1 antibody-conjugated magnetic beads.

How is cell purity estimated?

Primary endothelial cells produced from Creative Bioarray display typical spindle morphology/or cobblestone morphology under light microscopy and show VE-cadherin (CD144) or CD31 staining at cell-cell junction as demonstrated by fluorescence staining.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Good growth

Cells grow well in the medium recommended by the product.

09 Jan 2021

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