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Human Bone Marrow CD133+ Cells

  • Specification
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Cat.No.
CSC-C4574X
Description
Bone Marrow CD133+ Progenitor cells are positively isolated using a direct immunomagnetic CD133 MicroBead labeling system. The majority of CD133+ cells express the CD34 marker as well.
Species
Human
Source
Bone Marrow
Cell Type
CD133+; Progenitor
Disease
Normal
Storage and Shipping
Store in liquid nitrogen and ship in dry ice.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
  • Customer Review
Q:
What is CD133?
A:

CD133 (prominin-1) is a member of the transmembrane glycoprotein family and was initially described as a specific marker to select human hematopoietic progenitor cells. Later it was recognised as an important marker to identify and isolate the specific cell subpopulation termed “cancer stem cells” (CSCs). Many studies showed that CD133+ cells have stemness properties such as self-renewal, differentiation ability, high proliferation and they are also able to form tumors in xenografts.

Q:
How are CD133+ cells obtained?
A:

CD133+ cells are isolated using positive immunomagnetic cell separation from the bone marrow.

Q:
What is the purity of Creative Bioarray's CD133+ cells?
A:

Our CD133+ cells have been tested to have a purity of over 90% as measured by flow cytometry with a monoclonal CD133 antibody.

Q:
At what passage are the CD133+ cells upon arrival?
A:

Our CD133+ cells are cryopreserved directly after isolation. They haven’t been in culture before freezing.

Q:
Can I expand the CD133+ progenitors without differentiating them?
A:

No, the cells will proliferate and differentiate simultaneously upon being placed in culture. Varying the cytokine cocktail can influence the differentiation and proliferation of the cells.

Q:
Is it normal that CD133+ progenitor cells attach to tissue culture plastic after a week-long culture?
A:

The cells sink down to the bottom of the culture vessel but don’t really attach. They retain a roundish morphology and can be rinsed off with culture medium easily.

Q:
What happens if I grow CD133+ progenitors in hematopoietic progenitor cell expansion medium without cytokines?
A:

The cells will die. Hematopoietic progenitor cell expansion medium always must be supplemented with an appropriate cocktail of cytokines.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Good performance

Our group has purchased a number of cell products from Creative Bioarray, all of which have performed well.

01 Feb 2023


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