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HT-29

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C8201L
  • Tissue of Origin
  • Colon
  • Description
  • Established from an adenocarcinoma from a 44-year-old female. Tumorigenic in nude mice and has ability to grow in agar. They have human adrenergic alpha2A urokinase receptor (u-PAR), vitamin D (moderate expression) and urokinase receptor (u-PAR); The cell line can produce secretory component of IgA, carcinoembryonic antigen (CEA), transforming growth factor beta binding protein and mucin. Ultrastructural features include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough ER with free ribosomes, lipid droplets, few primary and many secondary lysosomes. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.
  • Organism
  • Homo sapiens, human
  • Morphology
  • epithelial
  • STR DNA Profile
  • Amelogenin: X,X
    CSF1PO: 11,12
    D13S317: 11,11
    D16S539: 11,12
    D5S818: 11,12
    D7S820: 10
    THO1: 6,9
    TPOX: 8,9
    vWA: 17,19
    D3S1358: 15,17
    D21S11: 29,30
    D18S51: 13
    Penta E: 14,16
    Penta D: 11,13
    D8S1179: 10
    FGA: 20,22
  • Culture Properties
  • adherent
  • Karyotype
  • The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.
  • Source
  • Colon
  • Quality Control
  • Tests for mycoplasma, bacteria and fungi were negative
  • Storage and Shipping
  • liquid nitrogen vapor phase
  • Application
  • HT-29 can be used as a suitable transfection host.
  • Disease
  • colorectal adenocarcinoma

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com