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HELA-S3

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C0306
  • Description
  • The cell line is a subclone of its parent HELA derived in 1955. This cell line can be adapted to grow in suspension; the cells are positive for keratin by immunoperoxidase staining. The HELA-S3 clone has been very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition, and plaque-forming ability.
  • Organism
  • Homo sapiens, human
  • Morphology
  • epithelial
  • STR DNA Profile
  • D3S1358: 15,18
    vWA: 16,18
    FGA: 18,21
    Amelogenin: X
    TH01: 7
    TPOX: 8,12
    CSF1P0: 9,10
    D5S818: 11,12
    D13S317: 13.3
    D7S820: 8,12
  • Culture Properties
  • Monolayer
  • Karyotype
  • Human flat-moded hypotriploid karyotype with 32% polyploidy - 68(53-68)<3n>XX, -X, +1, -2, -4, +5, +8, +12, -13, -14, -20, -21, -22, -22, +4mar - del(1)(p35), der(1)t(1;3) (p11;q11), der(3)t(3;5)(q11;q11), i(5p)x2, del(6)(q23), add(6)(q27), del(7)(q32).
  • Source
  • Cervix
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Application
  • The HELA-S3 cell line can be used for tumourigenicity, virus studies (poliovirus 1 and Ad 3) and clonal analysis of mammalian cell populations.

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    • USA
      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com