HCT-15

Specification
Recommended Products

Cat.No.

CSC-C0410

Description

Established from a colorectal adenocarcinoma; HCT-15 is a sister cell line of the cell line DLD-1. The HCT-15 cells are CSAp negative (CSAp-). They are positive for keratin by immunoperoxidase staining.

Species

Homo sapiens (human)

Tissue of Origin

Colon

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

Adherent

Morphology

epithelial

STR DNA Profile

Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19

Karyotype

Human pseudodiploid karyotype with 16% polyploidy - 46<2n>XYq+, t(8;17) (p23;q12/21), inv(11)(p14.2;q13) - stable karyotypic differences cf DLD-1 - matches published karyotype

Applications

The HCT-15 cell line can be used for tumourigenicity studies and also can be used as a suitable transfection host.

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -

Storage and Shipping

liquid nitrogen vapor phase

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.