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HCC-1937

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  • Cat.No.
  • CSC-C0522
  • Description
  • Established from a primary ductal carcinoma of the breast mammary gland from a 24-year-old Caucasian woman in 1995; cells were described to be homozygous for the BRCA1 mutation; a paired EBV+ B-lymphoblastoid cell line (B-LCL HCC-1937BL) is available
  • Recommended Medium
  • 85-90% RPMI-1640 + 10-15% h.i. FBS
  • Organism
  • Human
  • Morphology
  • adherent epithelial cells growing as monolayer, about 5-10% are giant vacuolated cells
  • Culture Properties
  • monolayer
  • Quality Control
  • Mycoplasma: negative in microbiological culture, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
    Viruses: PCR
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • breast carcinoma

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com