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GP5d
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Cat.No.
CSC-C9441J
Description
GP5d has been established from the same adenocarcinoma as GP2d. The cells were derived from a local recurrence of Duke's grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radio therapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess changes consistent with the usual pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP5d cells express relatively more TGF alpha mRNA than GP2d cells. GP5d cells undergo a marked epithelial-mesemchymal transition when treated with EGF.
Species
Human
Tissue of Origin
colon
Recommended Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Storage
Liquid Nitrogen (-180 °C).
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What is cell resuscitation?
A:
The process of restoring cell growth by thawing cells that have been frozen in liquid nitrogen or -70°C and then re-cultured. When restored to a normothermic state, the morphological structure of the cells remains normal and biochemical reactions can be restored.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Satisfied
I'm very satisfied with the services and products of various cell, and I look forward to working with them again in the future.
14 Mar 2022
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