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GP2d

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Cat.No.
CSC-C9456J
Description
GP2d has been established from the same adenocarcinoma as GP5d. This has been confirmed by STR profiling at. The cells were derived from a local recurrence of Duke's grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radiotherapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess genetic changes consistent with the pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP2d cells show growth response to EGF, TGF alpha or insulin with an increase in cell numbers. Amphiregulin mRNA is abundant in GP2d but almost undetectable in GP5d.
Species
Human
Tissue of Origin
colon
Recommended Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Storage
Liquid Nitrogen (-180 °C).
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-6317W
CSC-C0204
CSC-C0300
CSC-C0410
CSC-C0494
CSC-C0514

For research use only. Not for any other purpose.

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Q:
What are the types of vitamins in cell culture media?
A:

Biotin, folic acid, niacinamide, pantothenic acid, pyridoxine, riboflavin, thiamine, and vitamin B12 are all common components of culture media.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Incredible experience

I had an incredible experience with Creative Bioarray's cell culture products. Everything went smoothly and I was very satisfied with the results.

19 June 2022


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