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GOS-3

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  • Cat.No.
  • CSC-C0452
  • Description
  • Established from the tumor material of brain from a 55-year-old man with a mixed astro-oligodendroglioma (grade II/III); described to express neuroepithelial markers GFAP, vimentin and S-100 protein as well as pp60C-SRC, but not the neuro-specific variant pp60C-SRCN and to express neuropoietic factors CNTF, LIF and their receptors; LIF and oncostatin M induce growth inhibition and differentiation; DNA fingerprinting at Creative Bioarray showed clearly that this cell line is in reality a derivative of the human glioblastoma cell line U-343-MG
  • Recommended Medium
  • 90% DMEM + 10% h.i. FBS + 4 mM L-glutamine
  • Organism
  • Human
  • Morphology
  • fibroblastoid, adherent cells growing as monolayer
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypodiploid karyotype with 20% polypoidy - 44(40-45)<2n>XXY/XXYY, -6, +7, -10, -14, -22, der(1)del(1)(p21)t(1;9)(q42;q34), der(9)t(9;16)(p13;p11)t(1;9)(q42;q34), der(16)t(9;16)(p13;p11), der(18)t(6;18)(p12;q12.2) - gain of 7 and loss of 10 and 22 ty
  • Quality Control
  • Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
    Viruses: ELISA: rev
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • glioblastoma

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

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Tel: 44-207-048-3343
Email: info@creative-bioarray.com