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GMS-10

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  • Cat.No.
  • CSC-C0450
  • Description
  • Established from the malignant brain tumor of a 49-year-old man with glioblastoma in 1995
  • Recommended Medium
  • 85-90% DMEM + 10-15% h.i. FBS + 2 mM L-glutamine
  • Organism
  • Human
  • Morphology
  • fibroblast-like cells growing as monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Human flat-moded hypertriploid karyotype with 11% polyploidy - 69-76<3n>XXYY, +Y, +2, +3, +9, -10, -12, +15, +15, -18, +20, +20, -22, +2-4mar, +1-4dmin, del(2)(p11), add(2)(q31;hsr), der(4)t(3;4)(p21;p15.3), add(4)(p1?), del(6)(q23), add(7)(q11), add(9)(p
  • Quality Control
  • Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
    Viruses: ELISA: rev
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • glioblastoma

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com