GIRARDI HEART C2

Specification
Recommended Products

Cat.No.

CSC-C0283

Description

These cells were cloned from the GIRARDI HEART cell line (passage #623) in 1981 and were thought to "represent human heart cells"; however, isoenzyme analysis (Nelson-Rees et al., Science 191: 96-97, 1976) and DNA fingerprinting at the Creative Bioarray established unequivocally that this cell line must be considered de facto a subclone of HELA; cells were described to be sensitive to various toxins and hormones

Species

Human

Source

cervix carcinoma

Recommended Medium

45% SuperCult® Medium 199 (with Hanks salts) and 45% RPMI-1640 + 10% h.i. FBS.

Culture Properties

monolayer

Morphology

epithelial cells growing as monolayers

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HPV +, H

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1447Y

SuperCult® Medium 199

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CM-1448Y

SuperCult® RPMI 1640 Medium

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CS-001Y

PremSera® FBS

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For research use only. Not for any other purpose.