- Established from a bone marrow metastasis of a 42-month-old boy with stage IV neuroblastoma after 6 months of chemotherapy. IFN gamma inhibits growth of GIMEN. FGF-2 is antimitogenic for GIMEN cells, this antimitogenic effect of FGF-2 is reversed by IFN gamma and enhanced by IL-1 beta.
- Recommended Medium
- EMEM supplemented with 2 mM L-glutamine, 1% Non-Essential Amino Acids, 1mM sodium pyruvate and 10% fetal bovine serum.
- Homo sapiens ,human
- STR DNA Profile
- Amelogenin: X,X
Penta E: 15,20
Penta D: 9,13
- Culture Properties
- Quality Control
- Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, EpCAM -
- Storage and Shipping
- Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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