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GH4-C1
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Cat.No.
CSC-C1110
Description
Subclone of the cell line GH3; established from a pituitary tumor induced in a female Wistar/Furth rat in 1965; described to produce a high rate of prolactin and a low rate of growth hormone synthesis
Species
Rat
Source
pituitary tumor
Recommended Medium
80-90% Hams F10 + 10-20% h.i. FBS + 2 mM L-glutamine (originally grown with 15% horse serum + 2.5% h.i. FBS in Ham′s F10)
Culture Properties
suspension
Morphology
mostly round cells growing singly and in clusters in suspension and partially adherent; quality and amount of serum may influence the adherence of the cells
Karyotype
rat karyotype
Quality Control
Mycoplasma: contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, RNA hybridization, PCR assays
Viruses: PCR: SMRV -
Viruses: PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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