Fluorescent in situ hybridization (FISH) is a powerful technique
for detecting RNA or DNA sequences in cells, tissues, and
tumors. FISH provides a unique link among the studies of cell
biology, cytogenetics, and molecular genetics.
Creative Biolabs offers a full array of
custom Fluorescence in situ hybridization (FISH) service from
probe design, chromosome/cell preparation to expert result
interpretation. In situ hybridization is a technique to directly
identify a specific region of DNA or RNA in a cell.
Complementary probes linked to molecules such as biotin or
fluorescein can be used to visualize the target, which can be
chromosomes, interphase nuclei or tissue biopsy. This technology
is utilized for the characterization of chromosomal
rearrangements, chromosome locations, the oncogenes
amplification, and the detection of microdeletions.
Creative Biolabs also provide
Chromogenic In Situ Hybridization (CISH) service. CISH allows
detection of gene amplification, chromosome translocations and
chromosome number using conventional enzymatic reactions under
the brightfield microscope on formalin-fixed, paraffin-embedded
(FFPE) tissues.
A. |
Chromosome/Cell Preparation: We can help you
performing cell chromosome FISH (FISH on interphase,
metaphase and cultured cells), tissue chromosome FISH
(FISH on formalin-fixed, paraffin-embedded tissue or
cell slides) and RNA-FISH (FISH to study intracellular
RNA localization, RNA processing, quantitation). We
performed numerous tests aimed to improve the efficiency
of cytogenetic slide preparation and to increase FISH
signals. Several modifications of the general protocol
resulted in better chromosome spreading, better
chromosome morphology and shorter hybridization times,
while yielding brighter FISH signals. |
B. |
Probes Design, Labeling, Purification and Test:
Our FISH probes are synthetic pieces of DNA that couple
with a fluorescent indicator, so that the chromosomes or
genes that they bind to can be directly visualized under
our imaging analysis system. Our well-established
standard operating procedure allows efficient
optimization of labeling a variety of fluorescent
probes. Also, we can provide experimental services to
meet your special needs. Customized probes are available
as well. The choice of directly or indirectly labeled
SpectrumOrange, SpectrumGreen, or SpectrumAqua probes is
dependent upon the use and combination of other
fluorophores in the FISH assay. |
|
o Whole chromosome painting probes (WPP) |
o Chromosome arm painting probes (APP) |
o Chromosome terminal band painting probes
(TPP) |
o Chromosome enumeration probes (CEP) |
o Chromosome subtelomere probes (CSP) |
o chromosome loci specific probes (CLP) |
|
C. |
In Situ Hybridization: We can optimize the FISH
hybridization conditions and provide a full array of
FISH services. |
D. |
Result Interpretation: Our FISH imaging
analysis system consists of a fluorescent microscope, a
charge-coupled-device (“CCD”) camera and imaging
analysis software. All three components are provided by
our component suppliers with internal modifications.
Through the use of FISH probes, the imaging analysis
system enables medical practitioners to detect and
localize the presence or absence of specific DNA
sequences in chromosomes. |
E. |
Prenatal Diagnosis: Our optimized prenatal
diagnosis detection kits offer innovative technologies
and standard methods that are applied to the diagnosis
of fetal disorders. |
o Trisomy (21/18/13/12/9/8) |
o Turner Syndrome |
o Trisomy X |
o XYY Syndrome |
o DiGeorge Syndrome |
o Triploidy |
F. |
Cancer Diagnosis and Prognosis: Many cancer
diagnosis testing kits are available in our company. You
can also custom other cancer diagnosis probes according
to your needs. |
|
o Bladder Cancer |
o Breast Cancer |
o Cervical Cancer |
o Chronic Lymphocytic Leukemia |
o Chronic Granulocytic Leukemia |
o Multiple Myeloma |
|
G. |
Reagents for FISH: Our optimized reagents and
solutions will help you perform your FISH experiments
more smoothly and efficiently. |
H. |
Features: The advantages of our services and
products are as follows: |
|
o Simple, easy, fast and repeatable |
o Sensitive |
o Competitive price |
o easy to process multiple slides
simultaneously |
o High quality |
mRNA quantitative
determination
Fluorescence in situ
hybridization (FISH) could be used for low abundant mRNA
detection and advanced quantitative test.
Chromosome Localization and Amplification
Creative Biolabs will label and hybridize your DNA clones to
map the sub-chromosomal position of your gene on normal
metaphase chromosomes.
- Hybridize clones within a chromosomal
walk in two colors to interphase nuclei to determine
whether they are contiguous.
- Deletion/amplification studies of a
novel gene in tumor cell lines
- Novel gene or DNA fragment mapping on
banded chromosomes (human, mouse, and rat) .
- Analyze the number of gene copies
Transgene Analysis in
Transgenic Mice/Cell Lines
- Determine the transgene location
(within a specific chromosomal region)
- Analyze the number of integration sites
- Determined the relationship between a
transgene and specific genetic markers
- Determine the presence of homozygosity
Spectral Karyotyping
(SKY)
SKY is based on cohybridization of combinatorially labeled
chromosome-painting probes with unique fluorochrome
signatures onto human or mouse metaphase chromosome
preparations. Image acquisition and analysis use a
specialized imaging system, combining Sagnac interferometer
and CCD camera images to reconstruct spectral information at
each pixel.
Comparative Genomic Hybridization (CGH):
Comparative genomic hybridization (CGH) or Chromosomal
Microarray Analysis (CMA) is a competitive FISH assay that
can potentially demonstrate all regions of amplification and
deletion in the genome.
Chromogenic In Situ
Hybridization （CISH）:
CISH is a practical, cost-effective, and valid alternative
to fluorescent in situ hybridization (FISH) and can be
easily integrated into routine testing in laboratories.
 |
HER-2/neu oncogene
amplification by chromogenic in situ hybridization |
|
