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EGI-1

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C0433
  • Description
  • Established from the solid tumor of a 52-year-old Caucasian man with advanced stage malignant bile duct carcinoma (patient had not received any chemotherapy prior to cell explantation) in 1984 and three consecutive passages in female nu/nu mice (NMRI); primary tumor histology: large cell adenocarcinoma of low differentiation, metastasizing (ascites in patient); human character of cell line confirmed by karyotype in passage #34 of in vitro culture (1986)
  • Recommended Medium
  • 90% DMEM + 10% h.i. FBS + 1x MEM amino acids (both essential and non-essential) + 2 mM L-glutamine
  • Organism
  • Human
  • Morphology
  • adherent polymorphic (epitheloid or dendritric) cells growing in monolayers with multilayer foci
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypertriploid with 8% polyploidy - 70(68-72)<3n>XYY, -8, -10, +11, -12, -15, +21, +5mar, del(4)(q22), i(5q), i(6p)x2, add(18)(q23)x2, del(21)(q22) - rearrangements of 18q and 21q implicated in gall bladder carcinoma
  • Source
  • bile duct carcinoma
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
    Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vime
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com