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EFM-192C

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  • Cat.No.
  • CSC-C0388
  • Description
  • Established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; sister cell lines EFM-192A and -192B show differences in molecular aspects; erbB-2 gene is amplified and overexpressed
  • Recommended Medium
  • 80% RPMI-1640 + 20% h.i. FBS
  • Organism
  • Human
  • Morphology
  • epitheloid adherent cells growing as monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Highly rearranged human hypohexaploid karyotype with 10% polyploidy - 122-138<6n>XXXXX/XXXXXX - related to EFM-192A and EFM-192 B
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin -
    Viruses: EL
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • breast carcinoma

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com