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EFM-192A

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  • Cat.No.
  • CSC-C0358
  • Description
  • Established from the pleural effusion of the left side from a 46-year-old Caucasian woman with metastasizing breast adenocarcinoma in 1985; sister cell lines EFM-192B and C show differences in molecular aspects; erbB-2 gene is amplified and overexpressed
  • Recommended Medium
  • 80% RPMI-1640 + 20% h.i. FBS + 2 mM L-glutamine
  • Organism
  • Human
  • Morphology
  • epitheloid, adherent growing as monolayer
  • Culture Properties
  • monolayer
  • Karyotype
  • Human flat-moded near-triploid karyotype with 20% polyploidy - 68(60-70)<3n>XXXX, +X, -1, -4, +9, -12, -17, -17, -18, -22, +6mar, add(1)(p22), add(1)(p?32), add(2)(q35/37), add(3)(q27/29), i(15q), add(17)(p12), dminx1 present at 38%
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • breast carcinoma

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com