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EFM-19

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  • Cat.No.
  • CSC-C0338
  • Description
  • Established from the pleural effusion of a 50-year-old Caucasian woman with breast carcinoma (ductal type, histological grading II) in 1979
  • Recommended Medium
  • 90% RPMI-1640 + 10% h.i. FBS
  • Organism
  • Human
  • Morphology
  • epitheloid, growing in clusters at first, then as monolayer
  • Culture Properties
  • monolayer
  • Karyotype
  • Human highly rearranged hypotriploid karyotype with 5.8% polyploidy - 62(58-65)<3n>X, der(X), apparent ch 17 nullisomy and preponderance of marker chromosomes, e.g. add(1)(p11), idel(1)(q11q?22), i(1p), i(2p), add(4)(p11), add(4)(p11), etc. - resembes ori
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • breast carcinoma

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com