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ECV-304

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  • Cat.No.
  • CSC-C0387
  • Description
  • DNA profiling studies revealed that STR patterns of the endothelial line ECV-304 and the human bladder line T24 were very similar, suggesting that ECV-304 was a derivative of T24. Furthermore, ATCC karyotypes of the two lines show two shared-marker chromosomes. Combined, these results show that ECV-304 is indeed a derivative of T24, a line that was developed years earlier. It is important to emphasize that all stocks of ECV show similar properties.
  • Recommended Medium
  • Medium 199 supplemented with L-glutamine and 10% fetal bovine serum.
  • Organism
  • Homo sapiens (human)
  • Morphology
  • adherent growth as cobblestone monolayers
  • STR DNA Profile
  • Amelogenin: X,X
    CSF1PO: 12
    D13S317: 12
    D16S539: 9
    D5S818: 10
    D7S820: 10,11
    THO1:6
    TPOX: 8,11
    vWA: 17
    D3S1358: 16
    D21S11: 29
    D18S51: 16,18
    Penta E: 7,10
    Penta D: 11,15
    D8S1179: 14
    FGA: 17,22
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypertriploid karyotype with 6.7% polyploidy - 78(75-81)<3n>XX, -X, +1, -4, +5, +5, +5, +7, -8, -9, -10, +14, -15, -15, +17, +18, +20, +20, +20, +21, +22, +4-6mar - add(1)(p32)x1-2, i(1p), der(2q;5q), der(3)t(3;11)(p25;q13), i(5p)x2, inv(7)(p14q31)
  • Source
  • urinary bladder; carcinoma
  • Quality Control
  • Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
    specific PCR: negative
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • urinary bladder; carcinoma

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com