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  • Cat.No.
  • CSC-C1077
  • Description
  • Established from lung of a 7-month-old bovine fetus; useful for detection of Pasteurella multocida toxine
  • Recommended Medium
  • 85% RPMI-1640 or MEM (with Earles salts) + 15% h.i. FBS
  • Organism
  • Bovine
  • Morphology
  • rectangular-like adherent cells growing as monolayers
  • Culture Properties
  • monolayer
  • Karyotype
  • Apparently bovine karyotype with bimodal ploidy distribution - 42(40-42)<2n>/80(77-84)<4n> - structurally rearranged with apparent reduction of ch number by centric fusion followed by tetraploidization
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
    Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • embryonic lung cells

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com