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DU-4475

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  • Cat.No.
  • CSC-C0464
  • Description
  • Established from the local metastatic cutaneous nodule of an undifferentiated carcinoma of a 62-year-old woman which developped seven years after radical mastectomy of a poorly differentiated ductal carcinoma of the breast; cells were described to grow readily in athymic nude mice and to produce tumors from which cells could be introduced into culture in vitro; cells reportedly express components of milk fat globules and the BCL1 gene
  • Recommended Medium
  • 80% RPMI-1640 + 20% h.i. FBS
  • Organism
  • Human
  • Morphology
  • large round cells growing singly or in clusters in suspension
  • Culture Properties
  • suspension
  • Karyotype
  • Human flat-moded near-tetraploid karyotype with 12% polyploidy - 88-93<4n>XXXX, +1, +1, -5, -6, +9, -10, -10, +15, +15, -16, -16, +22, +4mar, i(1q)x2, ?add(1)(p35-36)x2, ?i(5p)x2, add(6)(p11), add(6)(p1?), del(6)(q25), add(9)(q35), del(11)(q24)x2, add(15)
  • Quality Control
  • Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 3.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • breast carcinoma

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com