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DT-40

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  • Cat.No.
  • CSC-C1127
  • Description
  • Established in 1985 from a bursal lymphoma, which was induced by infection of a 1 day old chicken with Rous-associated virus type 1 (RAV-1), an avian leukosis retrovirus carrying no cell-derived oncogene; cells are described to retain the ability to rearrange the immunoglobulin light chain gene (IgL) and to exhibit a high frequency of homologous gene targeting
  • Recommended Medium
  • 80% Advanced RPMI-1640 + 20% h.i. FBS + 1 mM sodium pyruvate + 2 mM L-glutamine + 4.5 g/L glucose + 50 μM mercaptoethanol
  • Organism
  • Chicken
  • Morphology
  • round cells growing in clumps of 10 to 100 cells in suspension
  • Culture Properties
  • suspension
  • Quality Control
  • Mycoplasma: negative in microbiological culture, PCR assays
    Viruses: SMRV -
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • B cell line

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com