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DK-MG

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  • Cat.No.
  • CSC-C0372
  • Description
  • Established from the glioblastoma multiforme (right parietal, at diagnosis) of a 67-year-old woman in 1991
  • Recommended Medium
  • 90% RPMI-1640 + 10% h.i. FBS + 2 mM L-glutamine
  • Organism
  • Human
  • Morphology
  • spindle, fibroblast-like cells growing mostly adherent as monolayer
  • Culture Properties
  • monolayer
  • Karyotype
  • Human hypodiploid karyotype with 12% polyploidy - 44(42-44)<2n>XX, +7, -9, -10, -12, -14, +mar, del(6)(q13q25), add(9)(p2?2), der(15)t(14;15)(q12;p11), del(16)(p13), del(17)(q25), add(19)(q1?3), variable numbers of dmin detectible in most cells - resemble
  • Quality Control
  • Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
    Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HI
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • glioma

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com