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COLO-824

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  • Cat.No.
  • CSC-C0259
  • Description
  • Established from the pleural fluid of a 52-year-old man with breast carcinoma in 1991. The cells do not tolerate DMSO; upon thawing, DMSO has to be removed by centrifugation.
  • Recommended Medium
  • RPMI-1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum.
  • Organism
  • Homo sapiens (human)
  • Morphology
  • Epithelial
  • STR DNA Profile
  • Amelogenin: X,X
    CSF1PO: 10,12
    D16S539: 13
    D13S317: 11,12,13
    D5S818: 12
    D7S820: 8,11
    THO1: 7,9
    TPOX: 6,11
    vWA: 16
    D3S1358: 16,17
    D21S11: 28
    D18S51: 15,19
    Penta E: 7
    Penta D: 5,10
    D8S1179: 12,14
    FGA: 22
  • Karyotype
  • Human highly rearranged hyperdiploid karyotype with 11% polyploidy - 51(48-52)<2n>X, -Y, +1, -2, -6, +7, -10, -13, -15, -19, -21, -22, +12mar, del(1)(p12), der(1)t(1;?)(p12;?), der(6)t(1;6)(q25;q12), der(7)t(7;?)(q32/34;?), der(8)t(8;?)(q24;?), der(12).
  • Source
  • Metastatis of a female breast cancer patient. (pleural effusion)
  • Quality Control
  • Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
    specific PCR: negative
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • Metastatis of a female breast cancer patient. (pleural effusion)

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Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com