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COLO-205

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-C8203L
  • Tissue of Origin
  • Colon
  • Description
  • This COLO 205 cell line was established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen. The cells are CSAp negative (CSAp-) and positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36,000 Dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen. This cell line can produce carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days, keratin and interleukin 10 (IL-10, interleukin-10).
  • Organism
  • Homo sapiens, human
  • Morphology
  • Epithelial
  • STR DNA Profile
  • Amelogenin: X,X
    CSF1PO: 11,12
    D13S317: 10,12
    D16S539: 12,13
    D5S818: 10,13
    D7S820: 9,10
    THO1: 8,9
    TPOX: 11
    vWA: 15
    D3S1358: 16
    D21S11: 30.2,33.2
    D18S51: 18
    Penta E: 13,15
    Penta D: 9,11
    D8S1179: 9,14
    FGA: 21,23
  • Culture Properties
  • suspension
  • Karyotype
  • The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic.
  • Source
  • Colon
  • Quality Control
  • Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
    specific PCR: negative
  • Storage and Shipping
  • liquid nitrogen vapor phase
  • Application
  • This cell line can be used as a suitable transfection host.
  • Disease
  • Colorectal adenocarcinoma; Dukes' type D

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com