CAL-85-1

Specification
Recommended Products

Cat.No.

CSC-C0474

Description

Established from the relapsing invasive galactophoric breast adenocarcinoma resected from a 35-year-old woman during radiotherapy in 1990; cells were described as expressing the multidrug resistance (MDR) gene

Species

Human

Source

breast adenocarcinoma

Recommended Medium

90% DMEM+ 10% h.i.FBS

Culture Properties

monolayer

Morphology

adherent epithelial-like cells growing in monolayers

Karyotype

Human flat-moded hypotriploid karyotype with 8% polyploidy - 60(57-65)<3n>XX/XXX, +2, +5, -8, -10, -12, -13, -14, -15, -16, -16, -17, -18, +?20, -21, -22, +2-4mar, +fra, add(X)(q2?), del(X)(q11), add(1)(p34), del(2)(q?11q?21)x1-2, add(2)(p2?3), der(3)?inv

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1010X

CS-001Y

For research use only. Not for any other purpose.